Tag

Part:BBa_M36981:Design

Designed by: Meghan Bowler, Nathan Barnett, Kian Torabian   Group: Stanford BIOE44 - S11   (2011-05-17)

Type II Signal Peptide (Correct)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 60
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 60
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 60
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 60
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This sequence is an N-terminus signal peptide of the major outer membrane lipoprotein, isolated from E.coli. Though there are variations, this was the first determined in 1977.

Source

E.coli

References

1. Protein Secretion: (SEC pathway): Oudega, B. (2003). Protein secretion pathways in bacteria. Dordrecht: Kluwer Academic Publishers.

2. Signal Peptide Sequence: Gennity, J., Goldstein, J., and Inouye, M. (1989) “Signal Peptide Mutants of Escherichia coli.” Journal of Bioenergetics and Biomembranes 22(3):233-269

3. Used to see if modified signal peptide could be predicted after codon optimization: Hiller K, Grote A, Scheer M, Munch R, Jahn D. (2004) “PrediSi: prediction of signal peptides and their cleavage positions.” Nucleic Acids Res 32:W375–379.